Quick fish sampling guide for disease diagnostics - Microbiome sampling guide

Abstract

Interactions between microbes associated with the host, other organisms present in the system and the environment itself are increasingly recognised to contribute to aquatic animal diseases. Those microbiome assemblages and their functions are extremely diverse and poorly understood. They vary over time in water and between host tissues and organs. High-throughput sequencing technologies (multi-omics) and bioinformatics analysis of complex microbiomes can provide biological insights offering new opportunities for early detection of pathogens and disease mitigation strategies. Similarly, such approaches are being applied to study the effect of locally available ingredients for use in fish feeds and their impact on fist gut microbiome and the health of the pond environment—optimising the growing conditions on-farm. WorldFish and partners developed this quick guide for microbiome sampling from fish skin/gills mucus swabs, internal organs (e.g. gut) and water samples from ponds, hatcheries tanks, river, canal etc.