Show simple item record

dc.creatorElgendy, M.en_US
dc.creatorAbdelsalam, M.en_US
dc.creatorMohamed, S.A.en_US
dc.creatorAli, S.E.en_US
dc.date.accessioned2022-09-11T19:27:31Z
dc.date.available2022-09-11T19:27:31Z
dc.date.issued2022en_US
dc.identifier.citationElgendy, M. Y. Abdelsalam, M. Mohamed, S. A. et al. Molecular characterization, virulence profiling, antibiotic susceptibility, and scanning electron microscopy of Flavobacterium columnare isolates retrieved from Nile tilapia (Oreochromis niloticus). Aquacult Int 30, 845–862 (2022). https://doi.org/10.1007/s10499-021-00819-xen_US
dc.identifier.issn0967-6120en_US
dc.identifier.issn1573-143Xen_US
dc.identifier.urihttps://hdl.handle.net/20.500.12348/5238
dc.description.abstractColumnaris is a common flavobacterial disease affecting tilapia aquaculture. Flavobacterium columnare has been identified as being responsible for the heavy mortalities of earthen-pond-cultured Nile tilapia (Oreochromis niloticus) at the Fayoum Governorate, Egypt. Mortalities have been closely associated with bad husbandry in the overstocked ponds. Diseased fish showed fin and tail rot with a thick yellowish turbid mucus covering the affected skin and gills. Scanning electron microscopy revealed the presence of numerous bacterial cells dispersed in the affected gill tissues. Most of the investigated specimens (60%) were infected with F. columnare. Forty-seven bacterial isolates were phenotypically identified based on cultural and biochemical characteristics. Molecular identification, virulence property assessment, and antibiotic-sensitivity testing were performed on 10 randomly selected isolates. The identities of the isolates were confirmed by gene sequence and phylogenetic analyses. These isolates yielded variable results regarding virulence genes (gtf, norB, and trx) and ability to adhere to fish gills. All isolates exhibited proteolytic and chondroitin lyase activities but had different antibiotic-sensitivity profiles. The pathogenicity of one highly pathogenic isolate was tested via intramuscular injection into juvenile O. niloticus. The challenged fish showed fin rot and skin ulceration with 80% cumulative mortalities. The study discussed critical points in the pathogenesis of columnaris disease affecting Nile tilapia that may help to find out effective control measures and refers to the need for prudent use of antimicrobials in aquaculture to protect aquatic animals and human health.en_US
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.rightsCopyrighted; all rights reserveden_US
dc.sourceAquaculture International;30,(2022) Pagination 845,862en_US
dc.subjectscanning electron microscopyen_US
dc.subjectcolumnaris diseaseen_US
dc.subjectFishen_US
dc.titleMolecular characterization, virulence profiling, antibiotic susceptibility, and scanning electron microscopy of Flavobacterium columnare isolates retrieved from Nile tilapia (Oreochromis niloticus)en_US
dc.typeJournal Articleen_US
cg.contributor.crpFISHen_US
cg.contributor.funderNorwegian Agency for Development Cooperationen_US
cg.contributor.projectIncreased Sustainability in the Aquaculture Sector in SSA, through improved Aquatic Animal Health Management (AHA)en_US
cg.coverage.countryEgypten_US
cg.coverage.regionNorthern Africaen_US
cg.subject.agrovocpathogenicityen_US
cg.subject.agrovocvirulenceen_US
cg.subject.agrovocmortalityen_US
cg.subject.agrovocnile tilapiaen_US
cg.contributor.affiliationHydrobiology department, Veterinary Division, National Research Centreen_US
cg.contributor.affiliationUniversity of Cairoen_US
cg.contributor.affiliationTiba Poultry Grandparentsen_US
cg.contributor.affiliationWorldFishen_US
cg.contributor.affiliationNational Research Centeren_US
cg.identifier.statusTimeless limited accessen_US
cg.identifier.ISIindexedISI indexeden_US
cg.contribution.worldfishauthorAli, S.E.en_US
cg.description.themeSustainable aquacultureen_US
dc.identifier.doihttps://dx.doi.org/10.1007/s10499-021-00819-xen_US
cg.creator.idShimaa El Sayed Mohamed Ali: 0000-0002-0227-8124en_US


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record