Conventional and molecular characterization of an Aeromonas isolate recovered from an aquaculture farm with high fish mortality in Kenya

cg.contribution.worldfishauthorDelamare-Deboutteville, J.en_US
cg.contributor.affiliationEgerton Universityen_US
cg.contributor.affiliationUniversity of Nairobien_US
cg.contributor.affiliationWorldFishen_US
cg.contributor.affiliationThe Norwegian Veterinary Instituteen_US
cg.contributor.affiliationNational Research Centeren_US
cg.contributor.affiliationKenya Medical Research Instituteen_US
cg.contributor.funderNorwegian Agency for Development Cooperationen_US
cg.contributor.projectIncreased Sustainability in the Aquaculture Sector in SSA, through improved Aquatic Animal Health Management (AHA)en_US
cg.coverage.countryKenyaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.idShimaa El Sayed Mohamed Ali: 0000-0002-0227-8124en_US
cg.creator.idJerome Delamare-Deboutteville: 0000-0003-4169-2456en_US
cg.description.themeAquacultureen_US
cg.identifier.statusOpen accessen_US
cg.subject.agrovocfishen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.subject.sdgSDG 3 - Good health and well-beingen_US
cg.subject.sdgSDG 12 - Responsible consumption and productionen_US
cg.subject.sdgSDG 14 - Life below wateren_US
dc.creatorMACHARIA, N.en_US
dc.creatorNjagi, L.en_US
dc.creatorMulei, I.en_US
dc.creatorNyaga, P.en_US
dc.creatorWanja, D.en_US
dc.creatorAli, S.E.en_US
dc.creatorDelamare-Deboutteville, J.en_US
dc.creatorB Kimemia, B.en_US
dc.date.accessioned2025-07-02T09:11:14Z
dc.date.available2025-07-02T09:11:14Z
dc.date.issued2025en_US
dc.description.abstractBacterial diseases can pose a significant challenge to aquaculture in Kenya, leading to significant economic losses. A fish farm in Narok County, Kenya, reported mass mortality among its reared fish which included Nile tilapia, catfish and ornamental fish. This study aimed to characterize an Aeromonas isolate recovered from the farm and assess its potential role in the outbreak. Water samples (n=10) were collected randomly from different points within the fish ponds using sterile universal bottles and transported to the bacteriology laboratory at the Department of Veterinary Pathology, Microbiology and Parasitology, University of Nairobi. The bacterial isolate was identified using conventional culture methods, biochemical assays, Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF), BD Phoenix machine tests, and Whole Genome Sequencing. Based on morphological, biochemical, and MALDI-TOF analyses, the isolate was identified as Aeromonas veronii and further confirmed as Aeromonas veronii biovar Sobria using the BD Phoenix system. The genome size of the isolate was approximately 4.6 Mb with a GC content of 59%. Phylogenetic analysis showed that the isolate clustered with fish isolates from South America and Asia and with human isolates from Asia and Australia. The study underscores the need for more studies on this bacterium’s pathogenicity and development of an autogenous vaccine for the prevention of its bacteriosis.en_US
dc.formatPDFen_US
dc.identifier.citationNDEGWA JOSEPH MACHARIA, Lucy W Njagi, Isaac R Mulei, Philip Nyaga, Daniel W Wanja, Shimaa Ali, Jerome Delamare-Deboutteville, Bryson B Kimemia. (1/1/2025). Conventional and molecular characterization of an Aeromonas isolate recovered from an aquaculture farm with high fish mortality in Kenya. International Journal of Fisheries and Aquatic Studies, 13 (1), pp. 1-9.en_US
dc.identifier.doihttps://dx.doi.org/10.22271/fish.2025.v13.i1a.3008en_US
dc.identifier.issn2394-0506en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12348/6517
dc.languageenen_US
dc.publisherAkiNik Publicationsen_US
dc.rightsCC-BY-NC-SA-4.0en_US
dc.sourceInternational Journal of Fisheries and Aquatic Studies;13,Pagination 1-9en_US
dc.subjectmorphologicalen_US
dc.subjectaeromonas veronii biovar sobriaen_US
dc.subjectbiochemical. maldi-tofen_US
dc.subjectphylogeneticen_US
dc.titleConventional and molecular characterization of an Aeromonas isolate recovered from an aquaculture farm with high fish mortality in Kenyaen_US
dc.typeJournal Articleen_US

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