Quick protocol for antimicrobial susceptibility testing (AST) in aquatic animal species from aquaculture and fisheries
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Antimicrobial Resistance (AMR) happens when microorganisms such as bacteria, viruses, fungi and parasites are able to resist the inhibition or killing effects of medicines used to treat the infections they cause, making drugs ineffective and microorganisms resistant. The misuse or, overuse of antibiotics to treat bacterial infections affecting aquaculture species can speed up the AMR process. This is caused by lack of veterinarian supervision and inadequate laboratory testing methods. WorldFish in close collaboration with Cefas researchers from the UK FAO Reference Centre for Antimicrobial Resistance developed this quick protocol for antimicrobial susceptibility testing (AST) in aquatic animal species from aquaculture and fisheries. This protocol is intended to support and benefit national partners and researchers working in Asia and Africa in the areas of aquatic food systems and One Health and bring harmonization of methodologies across ongoing initiatives.
Citation
Umme Laila Urmi, Shafiq Rheman, Jerome Delamare-Deboutteville, Vishnumurthy Mohan Chadag, Michelle Stone, David Verner-Jeffreys, Andy Powell. (10/9/2021). Quick protocol for antimicrobial susceptibility testing (AST) in aquatic animal species from aquaculture and fisheries[Guideline]. Malaysia: WorldFish (WF).
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Author(s) ORCID(s)
Jerome Delamare-Deboutteville https://orcid.org/0000-0003-4169-2456
Vishnumurthy Mohan Chadag https://orcid.org/0000-0002-2574-284X
Vishnumurthy Mohan Chadag https://orcid.org/0000-0002-2574-284X
Date available
2021
Type
Publisher
WorldFish (WF)
Language(s)
Keyword(s)
aquaculture
; training; gram positive bacteria; gram negative bacteria; disease; antimicrobial resistance (amr); antimicrobial use; gram staining; antimicrobial susceptibility testing (ast); disk diffusion method; clsi guideline; muller hinton agar (mha); antibiotic disks; bacteriological sampling; muller hinton broth (mhb); inhibition zone diameter; epidemiological cut-off values (ecv/ecoff); clinical breakpoints; non-wild type (resistant); wild type (susceptible); Fish
; training; gram positive bacteria; gram negative bacteria; disease; antimicrobial resistance (amr); antimicrobial use; gram staining; antimicrobial susceptibility testing (ast); disk diffusion method; clsi guideline; muller hinton agar (mha); antibiotic disks; bacteriological sampling; muller hinton broth (mhb); inhibition zone diameter; epidemiological cut-off values (ecv/ecoff); clinical breakpoints; non-wild type (resistant); wild type (susceptible); Fish