Growth and survival of olive barb, Puntius sarana (Hamilton 1822) larvae produced with cryopreserved versus fresh sperm

Abstract

Despite the success in fertilization and hatching of fish eggs with cryopreserved sperm, report on growth and survival of larvae produced from frozen-thawed sperm is inadequate. This study evaluates the applicability of cryopreserved sperm for mass seed production by comparing the growth and survival of a popular food-fish olive barb, Puntius sarana (Hamilton 1822) larvae produced from cryopreserved and fresh sperm.