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dc.creatorMabrok, M.en_US
dc.creatorElayaraja, S.en_US
dc.creatorChokmangmeepisarn, P.en_US
dc.creatorJaroenram, W.en_US
dc.creatorArunrut, N.en_US
dc.creatorKiatpathomchai, W.en_US
dc.creatorDebnath, P.P.en_US
dc.creatorDelamare-Deboutteville, J.en_US
dc.creatorChadag, V.en_US
dc.creatorFawzy, A.en_US
dc.creatorRodkhum, C.en_US
dc.date.accessioned2020-08-24T07:12:37Z
dc.date.available2020-08-24T07:12:37Z
dc.date.issued2020
dc.identifier.citationMabrok, M. et al. (2020). Rapid visualization in the specific detection of Flavobacterium columnare, a causative agent of freshwater columnaris using a novel recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) assay. Aquaculture, 531: 735780.en_US
dc.identifier.issn0044-8486en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12348/4311
dc.description.abstractFlavobacterium columnare is the causative agent of columnaris, a serious disease affecting numerous freshwater fish species worldwide. Although several molecular protocols have been established to detect this pathogen, there still remains a need to develop simpler on-field applicable techniques. Here, we report a more practical and efficient assay to detect F. columnare based on the combination of recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD). The assay, as performed for 30 min at 37 °C for RPA, followed by 2 min at ambient temperature for LFD, revealed a comparable detection limit (200 fg total DNA and 0.4 CFU) to the PCR assay developed by Mabrok et al. in 2020 but required only ~35 min to complete tests (i.e. ~four times faster). Cross-reactivity against other bacteria and false results on 20 clinical samples were not observed, indicating its extremely high specificity and accuracy. The robust performance of the assay to crude tissue samples and clinical specimens reflect its potential use in field application/low-resource settings. The use of a fast and affordable DNA extraction kit is imperative as part of our assay to make the detection of F. columnare more feasible.en_US
dc.formatPDFen_US
dc.languageenen_US
dc.publisherElsevieren_US
dc.rightsCC-BY-NC-4.0en_US
dc.subjectrapid diagnosticsen_US
dc.subjectrecombinase polymerase amplificationen_US
dc.subjectdisease detectionen_US
dc.subjectflavobacterium columnareen_US
dc.subjectfreshwater columnarisen_US
dc.subjectlateral flow dipsticken_US
dc.subjectportable assayen_US
dc.subjectfreshwater fishen_US
dc.subjectFishen_US
dc.titleRapid visualization in the specific detection of Flavobacterium columnare, a causative agent of freshwater columnaris using a novel recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) assayen_US
dc.typeJournal Articleen_US
dcterms.bibliographicCitationMabrok, M. et al. (2020). Rapid visualization in the specific detection of Flavobacterium columnare, a causative agent of freshwater columnaris using a novel recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD) assay. Aquaculture, 531: 735780.
cg.contributor.crpFISHen_US
cg.contributor.funderCGIAR System Officeen_US
cg.coverage.countryThailanden_US
cg.coverage.regionSouth East Asiaen_US
cg.identifier.worldfish4825
cg.subject.agrovocaquacultureen_US
cg.subject.agrovocdisease controlen_US
cg.subject.agrovocpathogensen_US
cg.subject.agrovocfishen_US
cg.subject.agrovocfish diseasesen_US
cg.contributor.affiliationNational Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnologyen_US
cg.contributor.affiliationSuez Canal Universityen_US
cg.contributor.affiliationMahidol University, Faculty of science, Center of Excellence for Shrimp Molecular Biology and Biotechnologyen_US
cg.contributor.affiliationChulalongkorn Universityen_US
cg.contributor.affiliationNational Science and Technology Development Agencyen_US
cg.contributor.affiliationWorldFishen_US
cg.identifier.statusTimeless limited accessen_US
cg.identifier.ISIindexedISI indexeden_US
cg.contribution.worldfishauthorDebnath, P.P.en_US
cg.contribution.worldfishauthorDelamare-Deboutteville, J.en_US
cg.contribution.worldfishauthorChadag, V.en_US
cg.description.themeSustainable aquacultureen_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.aquaculture.2020.735780en_US
cg.creator.idJerome Delamare-Deboutteville: 0000-0003-4169-2456en_US
cg.creator.idVishnumurthy Mohan Chadag: 0000-0002-2574-284Xen_US


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