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dc.creatorBartie, K.L.en_US
dc.creatorTaslima, K.en_US
dc.creatorBekaert, M.en_US
dc.creatorWehner, S.en_US
dc.creatorSyaifudin, M.en_US
dc.creatorTaggart, J.B.en_US
dc.creatorde Verdal, H.en_US
dc.creatorRosario, W.en_US
dc.creatorMuyalde, N.en_US
dc.creatorBenzie, J.en_US
dc.creatorMcAndrew, B.J.en_US
dc.creatorPenman, D.J.en_US
dc.date.accessioned2020-11-10T13:58:41Z
dc.date.available2020-11-10T13:58:41Z
dc.date.issued2020
dc.identifier.citationBartie, K. L., Taslima, K., Bekaert, M. et al. (2020). Species composition in the Molobicus hybrid tilapia strain. Aquaculture, 526: 735433.en_US
dc.identifier.issn0044-8486en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12348/4397
dc.description.abstractThe “Molobicus” hybrid breeding programme was initiated to improve tilapia growth performance in brackish water. The base population was created by backcrossing F1 Oreochromis niloticus GIFT strains × feral O. mossambicus to O. mossambicus and selective breeding conducted for performance in brackish water with two lines selected in extensive or intensive rearing conditions. A panel of ten diagnostic SNP markers was applied to estimate the species composition at different stages in the Molobicus programme including parental stocks, F1 and seventh generation fish from the selective lines. The O. aureus-specific markers tested revealed zero or negligible contribution from O. aureus to all the groups analysed. Feral O. mossambicus possessed an estimated 0.98 frequency of O. mossambicus-specific alleles, while GIFT samples had an estimated mean frequency of 0.88 O. niloticus-specific alleles. Hybrid F1 GIFT × feral O. mossambicus samples demonstrated close to 50:50 allele frequencies from O. niloticus and O. mossambicus for seven of the eight SNP loci tested. Analysis of the combined seventh generation Molobicus samples revealed a significant excess of O. niloticus alleles in six out of the eight SNPs tested, with this trend being more pronounced in the line selected in intensive culture conditions and showing increased body weight. PCR-based SNP assays such as these can be used to inform on the individual species contribution of fish stocks and provide tools for the genetic management of the tilapia species and future breeding programmes.en_US
dc.languageenen_US
dc.publisherElsevieren_US
dc.rightsCC-BY-NC-SA-4.0en_US
dc.sourceAquaculture;526,(2020)en_US
dc.subjectgiften_US
dc.subjectaquacultreen_US
dc.subjectspecies contributionen_US
dc.subjectmolobicusen_US
dc.subjectspecies-specific snp markersen_US
dc.subjectoreochromis mossambicusen_US
dc.subjectoreochromis aureusen_US
dc.titleSpecies composition in the Molobicus hybrid tilapia strainen_US
dc.typeJournal Articleen_US
cg.contributor.crpFISHen_US
cg.coverage.regionGlobalen_US
cg.subject.agrovocaquacultureen_US
cg.subject.agrovocbreedingen_US
cg.subject.agrovocgeneticsen_US
cg.subject.agrovoctilapiaen_US
cg.subject.agrovocoreochromis niloticusen_US
cg.subject.agrovocselective breedingen_US
cg.contributor.affiliationThe French Agricultural Research Center for International Developmenten_US
cg.contributor.affiliationWorldFishen_US
cg.contributor.affiliationUniversity of Stirlingen_US
cg.contributor.affiliationBangladesh Agricultural Universityen_US
cg.contributor.affiliationPhilippines Department of Agriculture, Bureau of Fisheries and Aquatic Resourcesen_US
cg.identifier.statusTimeless limited accessen_US
cg.identifier.ISIindexedISI indexeden_US
cg.contribution.worldfishauthorBenzie, J.en_US
cg.description.themeSustainable aquacultureen_US
dc.identifier.doihttps://dx.doi.org/10.1016/j.aquaculture.2020.735433en_US
cg.creator.idJohn Benzie: 0000-0001-9599-8683en_US


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