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dc.creatorElgendy, M.en_US
dc.creatorAbdelsalam, M.en_US
dc.creatorM. Kenawy, A.en_US
dc.creatorAli, S.E.en_US
dc.date.accessioned2022-09-11T19:13:21Z
dc.date.available2022-09-11T19:13:21Z
dc.date.issued2022en_US
dc.identifier.citationElgendy, M. Y. Abdelsalam, M. Kenawy, A. M. et al. Vibriosis outbreaks in farmed Nile tilapia (Oreochromis niloticus) caused by Vibrio mimicus and V. cholerae. Aquacult Int (2022). https://doi.org/10.1007/s10499-022-00921-8en_US
dc.identifier.issn0967-6120en_US
dc.identifier.issn1573-143Xen_US
dc.identifier.urihttps://hdl.handle.net/20.500.12348/5237
dc.description.abstractVibriosis is a common disease in aquaculture. Nile tilapia (Oreochromis niloticus) farmed within some commercial fish farms in Kafr El-Sheikh governorate Egypt showed signs of disease and exhibited heavy mortality. In order to get to the root of the problem, ninety moribund tilapia were sampled from the affected fish farms and subjected to bacteriological and molecular examinations. Biochemical characterization of bacterial isolates was performed via the API 20E analytical system. All examined fish samples were infected with Vibrio species. Eighteen Vibrio isolates, V. mimicus (n = 12), and V. cholerae (n = 6) were taken randomly for molecular characterization and further analysis. Isolates were genotyped via sequencing and alignment of the recA gene. Isolates possessed numerous virulence traits, including the production of hemolysins, proteases, lipases, and nucleases. The prevalence of ompU, vmh, vpsR, and flrA virulence genes in Vibrio strains was 61.1%, 66.6%, 27.7%, and 33.3 %, respectively. The blaTEM (55.5%), apHAI (50%), and qnrVC (27.7%) antibiotic resistance genes were recorded in Vibrio strains. All Vibrio isolates (100%) were resistant to ampicillin 10 μg and amoxicillin 30 μg, while they showed high sensitivity against florfenicol 30 µg (83.3%) and ciprofloxacin 5 µg (77.7%). Challenge experiments in Nile tilapia confirmed pathogenicity of the isolates. Fish showed symptoms of septicemia and high mortality was observed. Infections induced numerous histopathological alterations in diseased fish. This is the first report of V. mimicus outbreaks associated with mass mortality in Egyptian farmed Nile tilapia. The findings form the basis for future development of effective control and preventive measures against one of the most infectious pathogens that affect fish and humans.en_US
dc.languageenen_US
dc.publisherSpringer (part of Springer Nature)en_US
dc.rightsCopyrighted; all rights reserveden_US
dc.sourceAquaculture International;(2022)en_US
dc.subjectvirulence genesen_US
dc.subjectv. choleraeen_US
dc.subjectreca gene sequence analysisen_US
dc.subjectvibrio mimicusen_US
dc.subjectFishen_US
dc.titleVibriosis outbreaks in farmed Nile tilapia (Oreochromis niloticus) caused by Vibrio mimicus and V. choleraeen_US
dc.typeJournal Articleen_US
cg.contributor.crpFISHen_US
cg.contributor.funderNorwegian Agency for Development Cooperationen_US
cg.contributor.projectIncreased Sustainability in the Aquaculture Sector in SSA, through improved Aquatic Animal Health Management (AHA)en_US
cg.coverage.countryEgypten_US
cg.coverage.regionNorthern Africaen_US
cg.subject.agrovocnile tilapiaen_US
cg.subject.agrovochistopathologyen_US
cg.contributor.affiliationHydrobiology department, Veterinary Division, National Research Centreen_US
cg.contributor.affiliationUniversity of Cairoen_US
cg.contributor.affiliationWorldFishen_US
cg.contributor.affiliationNational Research Centeren_US
cg.identifier.statusTimeless limited accessen_US
cg.identifier.ISIindexedISI indexeden_US
cg.contribution.worldfishauthorAli, S.E.en_US
cg.description.themeSustainable aquacultureen_US
dc.identifier.doihttps://dx.doi.org/10.1007/s10499-022-00921-8en_US
cg.creator.idShimaa El Sayed Mohamed Ali: 0000-0002-0227-8124en_US


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